![]() The trademark holder is not affiliated with Bio-HeliX Co., Ltd. * SuperSignal West Atto is a registered trademark of Thermo Fisher Scientific. A two-fold dilution series was prepared using rhCRP (R& Systems. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system. Detection of Recombinant Human CRP Visualized with VisULite MAX ECL Western Blotting Substrate. WesternSure ECL Stripping Buffer for Chemiluminescent Westerns Removes primary and secondary antibodies from PVDF or nitrocellulose membranes Maintains. Identical blots were incubated with the Western substrate. Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly. Add the detection mix directly to the blot (protein side up). Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Product overview High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. Drain the excess buffer from the washed blots. ![]() Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using Chemlux SPX-600 Series digital imaging system.įigure 2. Rapid Immunodetection Procedures There are two types of protocols for immunodetection: Standard and rapid. The PVDF membranes were spotted with serial dilution HRP and probed with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000).
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